Module-1

1. Basic concept of working in Molecular Biology Laboratory
2. Good Laboratory Practices and General Safety Instructions
3. Principle and Handling of Laboratory Equipment
4. Principal, Standard operating procedure and application of spectrophotometer
5. Basics of Calculations (Molarity and Normality): Buffers and Reagent Preparation
6. Process of Sterilization Techniques
7. Preparation of Media

Module-2

1. Overview of genomic DNA and RNA
2. Overview of distinguishing features of prokaryotic and eukaryotic cells
3. Overview of differential leukocyte cells
4. Preparation of blood smear and differential staining of blood cells
5. Total red blood cell count using haematocytometer
6. Determine the blood group and Rh factor

Module-3

1. Extraction of genomic DNA from Bacteria
2. Extraction of genomic DNA from Plant
3. Genomic DNA isolation from Blood
4. Isolation & Purification of Plasmid DNA
5. Types of electrophoresis
6. Basic concept for preparation of Tank buffer (TBE/TAE) – 6X loading dye and ETBr solution
7. Qualitative Analysis of DNA-Agarose Gel Electrophoresis
8. Quantitative Estimation of the DNA through UV-VIS spectrophotometer / Photo Calorimeter

Module-4

1. RNA Isolation from Plant Sources
2. Denaturing Agarose Gel Electrophoresis of Isolated RNA
3. Quantitative Estimation of the RNA through UV-VIS spectrophotometer / Photo Calorimeter

Module-5

1. Basic concept of PCR
2. Primer Designing and Optimization of PCR
3. Hands-on performance of PCR in thermal cycler
4. Gel Electrophoresis of PCR Products with Molecular Markers,
5. Basic concept of Blotting Technique.
6. Performance of SDS-PAGE

Module-1

1. Basic concept of Recombinant DNA Technology
2. Good Laboratory Practices and General Safety Instructions
3. Principle and Handling of Laboratory Equipment
4. Principal, Standard operating procedure and application of spectrophotometer
5. Basics of Calculations (Molarity and Normality): Buffers and Reagent Preparation
6. Process of Sterilization Techniques
7. Preparation of Media

Module-2

1. Overview of genomic DNA and RNA
2. Extraction of genomic DNA from Bacteria
3. Isolation & Purification of Plasmid DNA
4. Types of electrophoresis
5. Basic concept for preparation of Tank buffer (TBE/TAE) – 6X loading dye and ETBr solution
6. Qualitative Analysis of DNA-Agarose Gel Electrophoresis
7. Quantitative Estimation of the DNA through UV-VIS spectrophotometer / Photo Calorimeter

Module-3

1. RNA Isolation from Plant Sources
2. Denaturing Agarose Gel Electrophoresis of Isolated RNA
3. Quantitative Estimation of the RNA through UV-VIS spectrophotometer / Photo Calorimeter

Module-4

1. Basic concept of PCR
2. Primer Designing and Optimization of PCR
3. Hands-on performance of PCR in thermal cycler
4. Gel Electrophoresis of PCR Products with Molecular Markers,
5. Basic concept of Blotting Technique.
6. Performance of SDS-PAGE

Module-5

1. Introduction about ligation
2. Perform T4 DNA Ligation of λ DNA restriction enzyme
3. Observe the efficiency of ligation reaction through Agarose gel electrophoresis
4. Transformation of Ligated Plasmid into Competent Cells
5. Screening of the Transformed Cells (Blue-White selection)
6. Perform Colony PCR for Screening of Transformed Cells
7. Perform restriction-digestion of Plasmid DNA by restriction endonucleases
8. Perform Agarose gel electrophoresis of digested plasmid DNA
9. Perform Southern Blotting Technique (Transfer of DNA to Membrane)
10. cDNA Preparation and Cloning
11. Basic concept of DNA sequencing

Module-1

1. Basic concept of Biochemistry and Metabolomics
2. Good Laboratory Practices and General Safety Instructions
3. Principle and Handling of Laboratory Equipment
4. Principal, Standard operating procedure and application of spectrophotometer
5. Basics of Calculations (Molarity and Normality): Buffers and Reagent Preparation
6. Process of Sterilization Techniques
7. Preparation of Media

Module-2

1. Basic concept of Identification of Carbohydrate, Protein and Fat
2. Determination of pH optima of amylase by pH meter
3. Protein isolation from Plant sample
4. Partial Purification of plant Protein by Ammonium sulphate Precipitation Method
5. Estimation of protein by Lowry method (Preparation of Standard Curve and Estimation)
6. Performance of SDS-PAGE

Module-3

1. Quantitative estimation of Carbohydrate (Preparation of Standard Curve and Estimation)
2. Estimation of Nucleic Acid (Preparation of Standard Curve and Estimation)
3. Principal of DNS reagent
4. Bacterial Plasmid Isolation & Quantification

Module-4

1. Estimation of specific activity of an enzyme (amylase)
2. Separation of amino acids – Thin layer chromatography
3. To perform the isoelectric precipitation of casein present in milk
4. Determination of Total Alkalinity of Water
5. Introduction and types of titration,
6. To Estimate the Saponification value in fats/oils,
7. Perform Biuretic reaction for detection of peptide bond

Module-5

1. Phytochemical Analysis of Plant Secondary Metabolites
2. Anti-microbial effects of phytochemical by Agar Well Diffusion Technique
3. Quantification of Total Serum Protein in Blood Sample
4. To Quantify Blood Sugar level in Human Blood Sample
5. Performance of Dot ELISA
6. Practical applications of the Fenton reaction

Module-1

1. Basic concept of Primary and Secondary immunization
2. Basic concept of antigen and antibody interaction
3. Good Laboratory Practices and General Safety Instructions
4. Principle and Handling of Laboratory Equipment
5. Principal, Standard operating procedure and application of spectrophotometer
6. Basics of Calculations (Molarity and Normality): Buffers and Reagent Preparation
7. Process of Sterilization Techniques

Module-2

1. Overview of genomic DNA and RNA
2. Overview of distinguishing features of prokaryotic and eukaryotic cells
3. Overview of differential leukocyte cells
4. Principle and application of microscope
5. Perform Microscopic differentiation of white blood cell of blood smear
6. Preparation of blood smear and differential staining of blood cells
7. Total red blood cell count using haematocytometer
8. Determine the blood group and Rh factor
9. Bleeding time calculation
10. Clotting time estimation

Module-3

1. Isolation of Serum and Plasma from Blood
2. Haemoglobin estimation from human blood sample
3. Quantitative estimation of proteins in blood by Follins Lowry’s method
4. Perform Single Radial Immunodiffusion (SRID) for quantitating the antigens
5. Perform Antigen-antibody interaction by Ouchterlony Double Diffusion Method

Module-4

1. Quantification of Total Serum Protein in Blood Sample
2. To Quantify Blood Sugar level in Human Blood Sample
3. Performance of Dot ELISA
4. Estimation of specific activity of an enzyme (amylase)
5. Determination of pH optima of amylase by pH meter
6. Application of RFLP (Restriction Fragment length Polymorphism)
7. Performance of SDS-PAGE

Module-1

1. Introduction about medical and clinical microbiology
2. Good Laboratory Practices and General Safety Instructions
3. Principle and Handling of Laboratory Equipment
4. Principal, Standard operating procedure and application of spectrophotometer
5. Basics of Calculations (Molarity and Normality): Buffers and Reagent Preparation
6. Process of Sterilization Techniques
7. Preparation of different types of media (Solid and Liquid Media)

Module-2

1. Identification and Classification of Microbes
2. Extraction of Genomic DNA from Microbes
3. Isolation and Screening of Microorganisms on the basis of Gram staining
4. Difference between gram-positive and gram-negative bacteria

Module-3

1. Serial Dilution techniques
2. Isolation of pathogenic bacteria (Pseudomonas sp., Vibrio sp., Salmonella sp., Staphylococcus sp., E. coli etc.)
3. Pure culture of pathogenic bacteria
4. Identification of Bacteria of clinical importance by various biochemical tests like
   • Catalase activity test
   • Oxidase activity test
   • Indole test
   • Citrate test
5. Plasmid DNA isolation from pathogenic bacteria
6. Different method for the detection of antimicrobial activity

Module-4

1. Antimicrobial effects of antibiotics against isolated pathogenic bacteria by disc diffusion
method
2. Antimicrobial effects of phytochemical by Agar Well Diffusion Technique
3. Performance of an Antigen with a set of Antibodies by Ouchterlony Double Diffusion Method
4. Basic concept of positive and negative controls
5. Minimum inhibitory concentration (MIC) via Agar diffusion method
6. Minimum bactericidal concentration (MBC) through Broth dilution method

Module-5

1. Overview of differential leukocyte cells
2. Preparation of blood smear and differential staining of blood cells
3. DNA Isolation from Blood Sample
4. Polymerase Chain Reaction (PCR) from Blood DNA
5. Gel Electrophoresis of PCR Products with Molecular Markers
6. Determine the Haemoglobin Content in Blood Sample
7. Quantify Blood Sugar level in Human Blood Sample
8. Performance of Dot ELISA

Module-1
1. Introduction about Industrial microbiology application & major products
2. Major companies in India: Scope & job prospects
3. Good Laboratory Practices and General Safety Instructions
4. Principle and Handling of Laboratory Equipment
5. Principal, Standard operating procedure and application of spectrophotometer
6. Basics of Calculations (Molarity and Normality): Buffers and Reagent Preparation
7. Process of Sterilization Techniques
8. Preparation of different types of media (Solid and Liquid Media

Module-2
1. Identification and Classification of Microbes
2. Serial Dilution techniques
3. Isolation of bacteria from soil
4. Isolation and Culturing of Microbes from Water Sample
5. Extraction of Genomic DNA from Microbes
6. Isolation of pure bacterial colonies
7. Slant Preparation & Sub Culturing of Microbes
8. Isolation and Screening of Microorganisms on the basis of Gram staining
9. Characterization of Isolated Bacteria by various biochemical tests like
   • Catalase activity test
   • Oxidase activity test
   • Indole test
   • Citrate test
10. Plasmid DNA isolation from Isolated bacteria

Module-3
1. Bacterial Growth Curve Analysis/Growth Kinetics
2. Optimization of growth condition for industrially important microorganisms
3. Effect of pH and temperature on microbial growth
4. Antibiotics Sensitivity Test (Agar disc diffusion method)
5. Minimum inhibitory concentration (MIC) via Agar diffusion method
6. Minimum bactericidal concentration (MBC) through Broth dilution method
7. Culturing Techniques for E. coli. or Industrially Important Microorganisms
8. Isolation of antibiotic producing microbe by crowed plate method

Module-4
1. Lactophenol Cotton Blue (LPCB) staining for fungus
2. Methylene Blue Reductase Test in Milk
3. Separation of Soy Protein by precipitation using salt
4. Production of wine from grapes
5. Separation of milk protein by Precipitation using organic acids
6. Isolation of Lactic acid bacteria from curd

Module-1
1. Introduction about food microbiology/biotechnology
2. Overview of adulteration and its disadvantages according to FSSAI guidelines
3. Good Laboratory Practices and General Safety Instructions
4. Principle and Handling of Laboratory Equipment
5. Principal, Standard operating procedure and application of spectrophotometer
6. Basics of Calculations (Molarity and Normality): Buffers and Reagent Preparation
7. Process of Sterilization Techniques
8. Preparation of different types of media (Solid and Liquid Media)

Module-2
1. Identification and Classification of Microbe
2. Serial Dilution techniques
3. Screening of diarrhea causing bacteria from spoil food sample
4. Determination of Moisture, Acidity and pH in food sample/beverages
5. Extraction of Genomic DNA from Microbes
6. Isolation of pure bacterial colonies
7. Slant Preparation & Sub Culturing of Microbes
8. Isolation and Screening of Microorganisms on the basis of Gram staining
9. Characterization of Isolated diarrhea causing bacteria by various biochemical tests like
   • Catalase activity test
   • Oxidase activity test
   • Indole test
   • Citrate test
10. Plasmid DNA isolation from Isolated bacteria

Module-3
1. Bacterial Growth Curve Analysis/Growth Kinetics
2. Optimization of growth condition for industrially important microorganisms
3. Effect of pH and temperature on microbial growth
4. Antimicrobial susceptibility test against diarrhea causing bacteria
5. Detection of Adulterants in spices powder, milk or honey

Module-4
7. Methylene Blue Reductase Test in Milk
8. Separation of Soy Protein by precipitation using salt
9. Production of wine from grapes
10. Separation of milk protein by Precipitation using organic acids
11. Isolation of Lactic acid bacteria from curd

Module-1

1. Basic concept of working in Research Laboratory
2. Good Laboratory Practices and General Safety Instructions
3. Principle and Handling of Laboratory Equipment
4. Basics of Calculations (Molarity and Normality)
5. Buffers and Reagent Preparation
6. Process of Sterilization Techniques
7. Basic principles, standard operating procedure (SOP) and application of instruments (Autoclave, pH meter, Laminar air flow, incubator, microscope and other  Microbiological Laboratory tools like Micropipettes, Petri plates, Spreaders, Inoculation Loop etc.).

Module-2

1. Overview of genomic DNA
2. Principal, Standard operating procedure and application of spectrophotometer
3. Types of extraction, Extraction of genomic DNA from Bacteria, Isolation & Purification of Plasmid DNA
4. Types of electrophoresis
5. Qualitative Analysis of DNA-Agarose Gel Electrophoresis
6. Quantitative Estimation of the DNA through UV-VIS spectrophotometer / Photo Calorimeter
7. Basic concept of PCR
8. Primer Designing and Optimization of PCR
9. Hands-on performance of PCR in thermal cycler.

Module-3

1. Basic concept of Identification of Carbohydrate, Protein and Fat
2. Estimation of protein by Lowry method (Preparation of Standard Curve and Estimation)
3. Estimation of specific activity of an enzyme (amylase)
4. Determination of pH optima of amylase by pH meter
5. Quantitative estimation of Carbohydrate
6. Standard graph preparation and data interpretation
7. Estimation of Nucleic Acid (Preparation of Standard Curve and Estimation)
8. Separation of amino acids – Thin layer chromatography
9. Perform Biuretic reaction for detection of peptide bond.

Module-4

1. Identification and Classification of Microbes
2. Isolation and Screening of Microorganisms on the basis of Gram staining
3. Preparation of different types of media (Solid and Liquid Media)
4. Difference between gram positive and gram negative bacteria
5. Serial Dilution techniques, Isolation of bacteria from soil
6. Isolation and Culturing of Microbes from Water Sample
7. Isolation of pure bacterial colonies
8. Slant Preparation & Sub Culturing of Microbes
9. Gram staining
10. Determination of antibiotic susceptibility of bacterial resistance.

Module-1
1. Introduction about Nano-Biotechnology and its application
2. Good Laboratory Practices and General Safety Instructions
3. Principle and Handling of Laboratory Equipment
4. Principal, Standard operating procedure and application of spectrophotometer
5. Basics of Calculations (Molarity and Normality): Buffers and Reagent Preparation
6. Process of Sterilization Techniques
7. Preparation of different types of media (Solid and Liquid Media)

Module-2
1. Basic principles, standard operating procedure (SOP) of magnetic stirrer with hot plate
2. Different methods for the preparation of nanoparticles
3. Synthesis of Silver-nanoparticles through chemical reduction methods
4. Synthesis of Gold-nanoparticles through chemical reduction methods

Module-3
1. Synthesis of Carbon quantum dots (CQDs) from plant sample
2. Characterization of Carbon quantum dots (CQDs)
3. Characterization of different nanoparticles through UV-Vis spectroscopy scanning method

Module-4
1. Screening of Medicinal plants for green synthesis of nanoparticles
2. Green synthesis of Ag- nanoparticles/Cu- nanoparticles/ Mg- nanoparticles through different medicinal plants
3. In-vitro Antimicrobial Activity of Green Synthesized Silver Nanoparticles of Leaf Extract against Bacterial Pathogens

4. DNA Protection Ability of Green Synthesized Nanoparticles

5. DNA Binding Ability of Green Synthesized Nanoparticles

Module-1

1.Introduction of waste water management and quality analysis.

2.Good Laboratory Practices and General Safety Instructions.

3.Principle and Handling of Laboratory Equipment.

4.Basics of Calculations (Molarity and Normality): Buffers and Reagent Preparation.

5.Process of Sterilization techniques.

6.Preparation of different types of media (Solid and Liquid Media).

Module-2

1.Overview of biological and chemical parameters of waste water samples.

2.Site visit and Sample (Waste water/ Treated water/ Harvested rain water) collection from different sites (domestic, STP, industry).

3.Sample preservation and preparation for further processing.

4.Analysis of physical parameters of the collected samples, i.e.- Turbidity, Temperature, Colour.

5.Analysis of biological oxygen demand (BOD-5) of the collected samples.

6.Overview and analysis of total coliform/ faecal coliform bacteria in waste water samples.

Module -3

1.Analysis of pH, conductivity, total suspended solids (TSS), total dissolved solids (TDS), total hardness of waste water samples.

2.Analysis of chemical oxygen demand (COD) of the collected samples.

3.Analysis of chemical parameters, i.e. Chloride, nitrates.

4.Analysis of total Kjeldahl nitrogen, ammoniacal nitrogen.

Module -4

1.Overview of SOP preparation.

2.Sop preparation of every biological and chemical parameter.

3.Comparative analysis of waste water samples from different sites.

Module-1
1. Introduction about Forensic Science and Forensic Agriculture
2. History of DNA fingerprinting: Ethics, Rules and Procedures
3. Scope of Forensic science and DNA fingerprinting in India
4. Major companies and Market size: Job prospects in India
5. Good Laboratory Practices and General Safety Instructions
6. Principle and Handling of Laboratory Equipment
7. Principal, Standard operating procedure and application of spectrophotometer
8. Basics of Calculations (Molarity and Normality): Buffers and Reagent Preparation
9. Process of Sterilization Techniques
10. Preparation of different types of media (Solid and Liquid Media)

Module-2
1. Genomic DNA isolation from human blood
2. Genomic DNA isolation from Hair
3. Genomic DNA isolation from saliva
4. Genomic DNA isolation from Nail
5. Qualitative Analysis of DNA-Agarose Gel Electrophoresis of isolated Genomic DNA,
6. Quantitative Estimation of isolated Genomic DNA through UV-VIS spectrophotometer / Photo Calorimeter

Module-3
1. PCR amplification of DNA using random primers for marker
2. Agarose gel electrophoresis of PCR products
3. Gel scoring and data analysis
4. RFLP Method: Genomic DNA isolation
5. Perform restriction-digestion of Genomic DNA by restriction endonucleases
6. Perform Agarose gel electrophoresis of digested plasmid DNA
7. Perform Southern Blotting Technique (Transfer of DNA to Membrane)
8. cDNA Preparation and Cloning
9. Basic concept of DNA sequencing

Module-4
1. Isolation of DNA from plant species
2. Quantification and quality assessment of isolated DNA using UV-VIS spectrophotometry
3. AFLP Method: DNA isolation

1. Basic concept of working in Research Laboratory
2. Good Laboratory Practices and General Safety Instructions
3. Basics of Calculations (Molarity and Normality): Buffers and Reagent Preparation
4. Process of Sterilization Techniques
5. Preparation of different types of media (Solid and Liquid Media)
6. Principle and Handling of all Laboratory Equipment
7. Aim, principle, Standard operating procedure (SOP) and Calibration of micropipette, reverse and forward pipetting
8. Aim, principle, SOP, Calibration and application of autoclave
9. Aim, principle, SOP, Calibration and application of of pH meter
10. Aim, principle and Calibration of lactometer
11. Aim, principle, SOP and application of soxhlet apparatus
12. Aim, principle, SOP and application of double distillation apparatus
13. Aim, principle, SOP and application of magnetic stirrer with hot plate
14. Aim, principle, SOP and application of TDS meter,
15. Aim, principle, SOP and application of vortex mixer;
16. Aim, principle, SOP, Calibration and application of Vertical laminar air flow
17. Aim, principle, SOP, Calibration and application of centrifuge
18. Aim, principle, SOP, Calibration and application of UV-VIS Spectrophotometer
19. Aim, principle, SOP, Calibration and application of photo calorimeter
20. Aim, principle, SOP, Calibration and application of Agarose gel electrophoresis
21. Aim, principle, SOP and application of PCR
22. Aim, principle, SOP, Calibration and application of SDS-PAGE
23. Aim, principle, SOP and application of microscope
24. Aim, principle, SOP and application of PCR
25. Aim, principle, SOP and application of TLC
26. Aim, principle, SOP and application Column chromatography
27. Aim, principle, SOP and application of ELISA

Module 1
1. Introduction to nanoscale measurements and characterization techniques
2. Understanding size-dependent phenomena and quantum effects
3. Fabrication methods for nanomaterials (top-down and bottom-up approaches)
4. Understanding interactions between biomolecules and nanomaterials

Module 2
1. Fabrication of nanoparticles using chemical and physical methods.
2. Spectroscopic analysis of nanomaterials (UV-Vis, fluorescence, etc.)
3. Characterization of functionalized nanoparticles (Zeta potential, FTIR, etc.)
4. Study of nanoparticle uptake and cellular imaging using microscopy techniques.

Module 3
1. Preparation of precursor materials (polymer, crosslinker, initiator).
2. Formation of nanogel through crosslinking reaction (e.g., polymerization).
3. Control of nanogel size and properties through reaction parameters.

Module 4

1. Removal of unreacted components using filtration or dialysis
2. Purification and modification characterization using spectroscopic techniques
3. Dynamic Light Scattering (DLS) for measuring nanogel size and size distribution.

Module 5

1. Importance of surface modifications and functionalization in nanobiotechnology
2. Viability assays (MTT, live/dead staining) to evaluate nanogel biocompatibility
3. Microscopy techniques (fluorescence, confocal) to visualize nanogel-cell interactions

Module 1

1. Introduction to Bioinformatics
2. Understanding Genomics Bioinformatics (database searching, information search, and data mining)
3. Databases & Tools (NCBI, UCSC, BLAST, etc) DNA/RNA/Protein sequence alignment.
4. Standalone BLAST Understanding for large-scale genomic data handling
5. Gene Prediction, Genome Annotation, Biological Functional Annotation

Module 2

1. Introduction to sequence alignment tools
2. Local Alignment
3. Pairwise Sequence alignment
4. Global sequence alignment
5. Multiple Sequence Alignment

Module 3

1. Sequence databases; Similarity matrices; Pairwise alignment
2. Features of dynamic Programming, alignment by Bayesian Statistical Methods,
3. multiple sequence alignment: local multiple sequence alignments: MEME, PSSM, HMM (algorithms and applications)
4. Progressive methods for global multiple sequence alignments: CLUSTALW, PILEUP, Statistical significance of alignment results
5. Consensus, regular expressions; PSSMs; Markov models; Regulatory sequence identification using Meme; Gene finding: composition-based finding, sequence motif-based finding.

Module 4

1. Introduction to Structure classification of proteins
2. SCOP (Structural Classification of Protein)
3. CATH databases
4. Basics of molecular Docking
5. Analysis of molecular docking and Protein-Ligand Docking
6. PCR Primer Designing

Module 5

1. RNA secondary structure prediction methods. Patterns, motifs, and Profiles in Sequences: Derivation and search methods. Gene prediction comparative phylogenetic analysis using MEGA, Functional genomics.
2. Protein Structure visualization, comparison, and classification
3. Protein Secondary Structure Prediction (globular protein, transmembrane protein, coiled-coil prediction)
4. Protein tertiary Structure Prediction method
5. Homology modeling, CASP, Threading and fold recognition.